Primer designing

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Published 2014-01-15

This RDT video discusses the properties required for designing a good primer for PCR
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All Comments (21)

@mceli87 9 years ago

I was studying this subject and couldn't understand why the reverse primer was the complement reverse of the leading strand. Now I got it, thanks to your awesome explanation. Thanks!!!
@zero450571 8 years ago

thank you so much. a 4 hour lecture in class did a horrible job of explaining what you explained so easily in 20 minutes. thank you
@shereenomer3391 3 years ago

You saved me as I am studying for the final examination... thank you so much
@adekunlemaryam1580 3 years ago

The way you explain this things Shomu. So helpful
@KamiiruRM 8 years ago

Thank you so much for this thorough explanation! It helps a lot.
@piko9311 10 years ago

Thank you very much for this informative tutorial! Congratulations on the good work!
@juliuskoome3055 2 months ago

Viewing from Kenya, with an example coming; you are really a saviour Sir.
@chakirahamada5689 10 years ago

Thank you very much. It's very clear. This RTD helps me a lot.
@hassank408 9 years ago

amazing..thanks a lot for making me understand this process for the first time.
@sychok958 4 years ago

Your explanation is better than my lecturer !!! Thank you so much, u save my final paper tmr !!!
@pezhman49 9 years ago

Thank you very much. It helps me understand this within 30 min.
@hyusup6794 10 years ago

Thank you very much. Very informative and helpful. Congratulation!
@aishaahmad4907 8 years ago

this is very good suman. it is the easiest way to explain primer design thank you
@natm1114 8 years ago

This was very helpful, thank you for sharing.
@sebastiandavidbuitragomora2351 1 year ago

Hello Shomu, thank you for your videos, I got a question, I designed a pair of primers to amplify one region of a 3200 bp gene. But after sequencing, some samples yield one section of the gene and others yield other section of the gene, do you have an explanation for this? thank you.
@kevajoseph5239 4 years ago

Dear Shomu, Thank you for the video above, it was quite helpful. I am having trouble designing new primers for HDV genotypes' G1-G8 and G1,2,4. How d I go about doing this? I have tried using NCBI.
@viniekouamou8188 6 years ago

Great Shomu. You are the best
@shanajparvin7218 8 years ago

Its really cool. thank you very much for helping others.
@AltafHussain-bg3db 1 year ago

Thank you sir i always see you lectures when cannot under stand the topics .thuk you so much. may God bless you with happiness and success in life.
@sublimetrance 5 years ago

I was wondering about the melting temperatures for the primers? For example, if one primer has a melting temperature(Tm) of 55 and another a Tm of 65. Say I lower the temperature to 60. Wouldn't the 65 Tm primer anneal but the temperature of 60 is still too high for annealing? So if I want both primers to anneal, don't I have to lower the reaction temperature to the lower of the two melting temperatures to get BOTH primers to anneal?