Primer Design, Validation & In-silico PCR in 5 minutes | AmplifX | Lecture 91 | Dr. Muhammad Naveed

Dr. Muhammad Naveed
Dr. Muhammad Naveed
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Published 2023-03-17
The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides. The primer therefore serves to prime and lay a foundation for DNA synthesis. In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified, resulting in millions and millions of copies in a very short timeframe. Primers are also used in DNA sequencing and other experimental processes.
AmplifX is a program dedicated to Polymerase Chain Reaction (PCR) experiments. The three main features are: Managing primers: keep all the informations for your hundreds or thousands of primers into a single database.

About Dr. Muhammad Naveed
(HoD, Biotechnology, University of Central Punjab, Lahore)

With distinction, Dr. Muhammad Naveed obtained a Ph.D. degree in Biotechnology (Genomics & Bioinformatics) from Quaid-e-Azam University, Islamabad. He has won Ph.D. indigenous & IRSIP scholarships from HEC. He has done Pre-Doc research at the University of Ghent, Belgium. HEC awarded him the best Ph.D. (IRSIP) Scholar of the year in 2013 & QAU honored him as a “Distinguished Alumni” in 2017. He is doing research projects in Bioinformatics, Molecular Biotechnology & Vaccine designing, and Drug designing against infectious diseases. He has supervised 70 MSc. and 60 MPhil. & 01 Ph.D. students. He has published 112 Research articles with 650 impact factors, 3000 citations, 01 book, and 03 book chapters. He was awarded the distinguished “Researcher of the Year” in 2016 (UoG) and 2018, 2019 & 2021 (UCP).
#primer #pcr #primervideo
All Comments (3)
  • @johirislam8174
    Hellow sir, I have a gene og 9500bp. How can I design primer . Does the prime should be designed for 9500bp? as you have shown in your this tutorial. If so then when i go for the pcr does the whole 9500bp gene become amplified?
  • @rimsharashid1279
    I obtained a separate gene sequence from ncbi than the one in the video. Then i followed all the steps of this video on Amplifx. Even thought my primer was designed smoothly, the primer was not validated. Why is that?