How to design pcr primers using NCBI primer blast

I hope this is more useful for the primer design. But if possible can u elaborate the information regarding the range and information about the GC content, self complimentary!!! It will be more helpful Thankyou very much

Very helpful and informative video sir .. thanku very much

A very good way for understanding.thanks

Thank you sir for this video, very helpful 🙏

thanks alot it really helps me alot

Sir make detail videos on ARMS and RfLp pcr primer design with detailed explanation

Thanks a lot for your wonderful videos. I have a request for videos on metagenomics data analysis in details, machine learning, R and Python. Thanks.

Very informative

Love your channel and the way you make difficult concepts easy. I had a question about primer property checking using Primer-BLAST. I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast… However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence” So that forced me to replace all the degenerate/wobble letters with N. Would that still make the property results correct? Am I still getting the right answers if they are replaced with N? Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.

My primers shows unintended similarity with other Genes. How to get rid off and get primer specific to gene???

thank you sir

How to validate the MSP Primers before ordering them and can we do the 5 gene methylation in a single tube? can anyone explain about how to carry out?

Nice explanation sir thank you🎉

How to download the designed primer

After selecting F primer, when i press Control to select R primer , the F selection is deselected. please guide

Very informative