Does Caramelization Effect Liquid Culture Growth?

One of, if not the most informative mycology channels on YT. Every expirement is done presicely and backed by actual results. Not just regurgitating the same stuff we hear from everyone else.

this was straight up scientific method. legend gary as always

I too have caramelised my LC by mistake, there was no noticeable difference except for a less clear solution, we sterilise our LC for 25 minutes at 15psi. It works for us!!!

Great experiment . Thanks for sharing this. I almost threw my LC out recently when I saw the particulates,but decided to inoculate as an experiment too. And then here's your timely video, giving me this good news. Thanks Gary .

beautiful experiment bro, thank you very much for your time !

Great experiment...but a more interesting one would be with different medias like dextrose, fructose, corn syrup, honey, and malt extract just to name a few, and mix in some peptone to make it interesting. Thanks again for your content.....LC videos are major views.

a sample size of "one" is not a truly scientific experiment. I PC my nutrient solutions for 35 to 45 minutes @15psi using clear, high fructose corn syrup. i never get solid caramelization, only a colour change. during my experiments with caramelization using honey, lme or dextrose I've found no concernable differences if caramelization happens or not in the 40 strains I have tested with a sample size of 6 with caramelization & 1 without caramelization of each of the 40 strains. we are all aware unless you are using 2 monokayorons to make your master culture you are getting many different genetics in a single culture which will lead to highly erratic experiment results. even using 2 monokayorons to make your master culture can lead to erratic experiment results because not every culture grows at the same rate in or on the same medium.

Nice work, real science 🎉

Thanks for the info I was looking for this information because I had this exact problem not on a large scale like yourself, although I am trying to maximize my lions mane production so all the help I can get is appreciated. I’d really like to know how to make a tincture from different fungi do you have a video of that?

Agreed, I'v always figured since caramelization is just the burt sugars and protiens and the myco consumes both then its going to consume it reguardless of it's form, but i did note that some strains/species do really well in caramelized lc and some dont...bottem line..da mushys WILL grow in a caramelized LC period...by the way @Felix Merz..I copy and pasted ur "caramelization" cuz I was'nt sure how to spell it..😆🤣😅

Perfect timing. I was about to throw out a batch of LC because of caramelization, I'm definitely keeping it!

have you tried to use your grain water for cultures and agar? I collected a bit of popcorn grain water from making spawn bags to run some side be side tests with 4% corn syrup solution.

Such an important experiment for any grower, glad someone is talking the time to do it!

I do mine for fifteen mins in my PC. But i only do one or two jars at a time. When it's empty like that you don't need to leave it in for all that long i have found. Also i fill my water line to match almost match the water level inside the jars and that helps a lot.

Master Gary the one who taught me about turbidity!!! Always top notch mush love ❤️

Keep dropping these bangers🍄 this information makes my brain tingle🧠⚡️I think it but you do it❤.

I am absolute beginner, this is my first LC but: I can use a snickers as "external stiring bar" because my Lion's Mane swims after it. Every other day it asks for KFC or some McD's. Fried chiccan AND caramelized topping for the icecream. Maybe brown rice instead of french fries - because it wants to remain lean. To still fit in a trunk. But no diet coke, yeah?!

Hey Gary! what is that pad you have in front of your flowhood @ 7:00?

What's the word on the morchella grow?

As always great content Gary!!! Are your syringe filters 25mm? I have some 11mm, same size as the SHIPs. Thanks!! Mush ❤